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KMID : 0900919980220040375
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Korean journal of Animal Reproduction
1998 Volume.22 No. 4 p.375 ~ p.383
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Effect of beta -Mercaptoethanol and Cysteamine on In Vitro Maturation of Porcine Follicular Oocytes
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Han Man-Hee
Park Byung-Kwon
Park Chang-Sik
Seo Kil-Woong
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Abstract
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This study was conducted to investigate the effect of beta-mercaptoethanol (beta-ME) and cysteamine on in vitro maturation of porcine follicular oocytes. The results obtained were summarized as follows : 1. When the immature oocytes were cultured at 0, 10, 50, 100 and 200 muM of beta-ME for 36h, the GVBD rates were 91.6, 92.5, 91.8, 91.9 and 92.7%, respectively, and the maturation rates of the oocytes with metaphase-II were 49.6, 41.7, 32.5, 34.1 and 35.4%, respectively. Thus, lower maturation rate was shown in beta-ME treated groups of 50, 100 and 200 muM as compared to control (non-treated) group (P＜0.05). After 44h of culture in the same treatments of beta-ME, the GVBD rates of porcine oocytes were 91.8, 90.4, 92.5, 91.2 and 93.9%, respectively, and the maturation rates were 71.9, 58.8, 56.7, 62.2 and 56.5% respectively. All treated groups of beta-ME showed lower maturation rates than the control group (P＜0.05) 2. When the immature oocytes were cultured at 0, 10, 50, 100 and 200 muM of cysteamine for 36h, the GVBD rates were 90.6, 86.3, 88.2, 87.2 and 90.0%, respectively, and the maturation rates were 53.8, 45.1, 54.4, 57.5 and 63.3% respectively. Especially, the maturation rate of 200 muM treated group was significantly higher than those of control group (P＜0.05). After 44h of culture in the same treatments of cysteamine, the GVBD rates of porcine immature oocytes were 89.5, 93.1, 85.1, 89.8 and 91.3%, respectively, and the maturation rates of the oocytes with metaphase- II were 84.2, 77.6, 66.0, 67.8 and 78.3%, respectively. Especially, the maturation rates of 50 and 100 muM treated groups were significantly lower than those of control group (P＜0.05).
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KEYWORD
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Porcine oocytes, IVM, beta-Mercaptoethanol (beta-ME), Cysteamine
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